Possible causes and measures for handling the phenomenon
Droplet instability/jumping. If there are bubbles in the sheath fluid filter, perform manual defoaming in Chapter 5 and repeat if necessary
Chip blockage: If the sample is not filtered or the particle diameter is too large to clear, a new chip needs to be replaced; Ensure that all samples pass through the filter element
Automatic calibration failed due to expired calibration beads, insufficient mixing, or improper temperature. Prepare fresh calibration beads (≤ 7 days) and shake the mixture thoroughly
Sort out abnormal deflection plates that are dirty or damp. Clean the deflection plates thoroughly and dry them thoroughly; Check the voltage setting of the deflection plate
The pressure cannot be established. The sheath fluid tank is not properly sealed or the compression pump is not turned on. Check the sealing ring of the tank cover; Confirm that the power supply of the compression pump is turned on and the pressure gauge shows 6 bar
Chapter 12: Summary of Daily Maintenance Points
Before daily start-up: check the sheath fluid, waste liquid, DI water tank, and clean the sorting room.
After each sorting, strictly follow the shutdown cleaning process and do not skip it.
Weekly: Check the validity period of the calibration beads and replace them in a timely manner.
Monthly: Check the liquid flow pipeline for leaks, clean the instrument surface and fan filter.
Every six months: Deep maintenance is performed by facility professionals, including laser alignment, PMT calibration, and pipeline replacement.
